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Image Search Results
Journal:
Article Title: Persistent inflammation induces GluR2 internalization via NMDA receptor-triggered PKC activation in dorsal horn neurons
doi: 10.1523/JNEUROSCI.4514-08.2009
Figure Lengend Snippet: NMDARs and PKC couple to AMPAR complex in dorsal horn. (A) PICK1 co-immunoprecipitates with GluR2 and PKCα (but not PKCβ or PKCγ). (B) GluR2 co-immunoprecipitates with PICK1 and PKCα. (C) PSD-95 co-immunoprecipitates with NR2B and stargazin. (D) Stargazin co-immunoprecipitates PSD-95 and GluR2. (E) NR1 (5-nm gold, arrowheads) and GluR2 (15-nm gold) co-localize at superficial dorsal horn synapses. Pre, presynaptic terminal; Post, postsynaptic structure. Scale bar: 100 nm
Article Snippet: Antibodies The following antibodies were used: rabbit anti-GluR2 (Chemicon, Temecula, CA), mouse anti-GluR2 (Chemicon), rabbit anti-GluR2-p Ser 880 ( Chung et al., 2003 ; Steinberg et al., 2006 ), rabbit ant-PICK1 ( Steinberg et al., 2006 ), rabbit anti-GRIP1 (Upstate, Lake Placid, NY),
Techniques:
Journal:
Article Title: Persistent inflammation induces GluR2 internalization via NMDA receptor-triggered PKC activation in dorsal horn neurons
doi: 10.1523/JNEUROSCI.4514-08.2009
Figure Lengend Snippet: Proposed model for the NMDAR/PKC-dependent dorsal horn GluR2 internalization under persistent inflammatory pain conditions. NMDARs couple to AMPARs through PSD-95 (which binds to NR2A/2B) interaction with stargazin (which binds to GluR1, GluR2, and GluR4). Under normal conditions, ABP/GRIP binds to and anchors GluR2 at synapses. Under persistent inflammatory pain conditions, NMDAR activation causes Ca2+ influx and PKCα activation. The latter phosphorylates GluR2 at Ser880 and disrupts GluR2 binding to ABP/GRIP, which leads to GluR2 internalization. GluR2 internalization results in an increase of AMPAR Ca2+ permeability. The increase in [Ca2+]i in dorsal horn neurons should initiate or potentiate a variety of Ca2+-dependent intracellular cascades that are associated with the maintenance of persistent inflammatory pain.
Article Snippet: Antibodies The following antibodies were used: rabbit anti-GluR2 (Chemicon, Temecula, CA), mouse anti-GluR2 (Chemicon), rabbit anti-GluR2-p Ser 880 ( Chung et al., 2003 ; Steinberg et al., 2006 ), rabbit ant-PICK1 ( Steinberg et al., 2006 ), rabbit anti-GRIP1 (Upstate, Lake Placid, NY),
Techniques: Activation Assay, Binding Assay, Permeability